AMFR antibody

Catalog No. ABIN7111885

This Rabbit Polyclonal antibody specifically detects AMFR in WB, IHC, ELISA, IP and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AMFR antibody (ABIN7111885)

Target: AMFR (Autocrine Motility Factor Receptor (AMFR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-AMFR Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: autocrine motility factor receptor

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IF: 1:10-1:100, IHC: 1:20-1:200, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for AMFR

Target: AMFR (Autocrine Motility Factor Receptor (AMFR))

Alternative Name: AMFR

Background: Synonyms:AMF receptor, isoform 2, AMFR, GP78, RING finger protein 45, RNF45 Background:E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation(ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.

Molecular Weight: 73 kDa

Gene ID: 267

UniProt: Q9UKV5

Pathways: ER-Nucleus Signaling