CD14 antibody

Catalog No. ABIN7112441

The Rabbit Polyclonal anti-CD14 antibody is suitable to detect CD14 in samples from Human, Mouse and Rat. It has been validated for FACS, WB, IHC, IF and ELISA.

Quick Overview for CD14 antibody (ABIN7112441)

Target: CD14

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-CD14 Antibody

Purpose: CD14 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: CD14 molecule

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IHC: 1:100-1:400, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background:

Synonyms: Monocyte differentiation antigen CD14|Myeloid cell-specific leucine-rich glycoprotein|Monocyte differentiation antigen CD14, urinary form|Monocyte differentiation antigen CD14, membrane-bound form|CD14

Background: Coreceptor for bacterial lipopolysaccharide(PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide(LPS)(PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response(PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway(PubMed:16880211).

Molecular Weight: 50-55 kDa

Gene ID: 929

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades