CDC25B antibody

Catalog No. ABIN7112566

This Rabbit Polyclonal antibody specifically detects CDC25B in WB, ELISA, IHC and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for CDC25B antibody (ABIN7112566)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-CDC25B Antibody

Purpose: CDC25B antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: cell division cycle 25 homolog B(S. pombe)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:1000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B

Background:

Synonyms: M-phase inducer phosphatase 2|Dual specificity phosphatase Cdc25B|CDC25B|CDC25HU2

Background: CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).

Molecular Weight: 60-70 kDa

Gene ID: 994

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy