DNMT3A antibody

Catalog No. ABIN7113542

The Rabbit Polyclonal anti-DNMT3A antibody is suitable to detect DNMT3A in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IP.

Quick Overview for DNMT3A antibody (ABIN7113542)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-DNMT3A Antibody

Purpose: DNMT3A antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: DNA(cytosine-5-)-methyltransferase 3 alpha

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNMT3A

Background:

Synonyms: DNA (cytosine-5)-methyltransferase 3A (Dnmt3a)|Cysteine methyltransferase DNMT3A|DNA methyltransferase HsaIIIA (DNA MTase HsaIIIA, M.HsaIIIA)|DNMT3A

Background: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3(H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

Gene ID: 1788

UniProt: Q9Y6K1