EIF3E antibody

Catalog No. ABIN7113947

The Rabbit Polyclonal anti-EIF3E antibody is suitable to detect EIF3E in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for EIF3E antibody (ABIN7113947)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EIF3E Antibody

Purpose: EIF3E antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: eukaryotic translation initiation factor 3, subunit E

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E

Background:

Synonyms: Eukaryotic translation initiation factor 3 subunit E (eIF3e)|Eukaryotic translation initiation factor 3 subunit 6|Viral integration site protein INT-6 homolog|eIF-3 p48|EIF3E|EIF3S6|INT6

Background: Component of the eukaryotic translation initiation factor 3(eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex(43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay(NMD), may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Molecular Weight: 48-52kd

Gene ID: 3646

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C