FBXW5 antibody

Catalog No. ABIN7114041

The Rabbit Polyclonal anti-FBXW5 antibody (ABIN7114041) specifically detects FBXW5 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FBXW5 antibody (ABIN7114041)

Target: FBXW5 (F-Box and WD Repeat Domain Containing 5 (FBXW5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-FBXW5 Antibody

Purpose: FBXW5 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: F-box and WD repeat domain containing 5

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for FBXW5

Target: FBXW5 (F-Box and WD Repeat Domain Containing 5 (FBXW5))

Alternative Name: FBXW5

Background:

Synonyms: F-box/WD repeat-containing protein 5|F-box and WD-40 domain-containing protein 5|FBXW5|FBW5

Background: Substrate recognition component of both SCF(SKP1-CUL1-F-box protein) and DCX(DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. The SCF(FBXW5) complex also mediates ubiquitination and degradation of actin-regulator EPS8 during G2 phase, leading to the transient degradation of EPS8 and subsequent cell shape changes required to allow mitotic progression. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B(IL1B) signaling pathway.

Molecular Weight: 65 kDa

Gene ID: 54461

UniProt: Q969U6