Bcl-2 antibody (AA 161-239)

Catalog No. ABIN707156

The Rabbit Polyclonal anti-Bcl-2 antibody has been validated for WB, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect Bcl-2 in samples from Human, Mouse, Rat, Pig, Rabbit and Sheep. There are 7+ publications available.

Quick Overview for Bcl-2 antibody (AA 161-239) (ABIN707156)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 161-239

Cross-Reactivity: Human, Mouse, Pig, Rabbit, Rat, Sheep

Predicted Reactivity: Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Bcl-2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Bcl-2 antibody (ABIN707156)

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Wang, Zhao, Yu, Feng, Zhang, Kou, Chu, Cui, Li, Zhang, Shen, Min: "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." in: Toxicology and applied pharmacology, Vol. 313, pp. 149-158, (2016) (PubMed).

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, (2016) (PubMed).

Huang, Zou, Shi, Zhang, Peng, Zhang, Gao, Wang, Zhang: "Electroacupuncture stimulates remodeling of extracellular matrix by inhibiting apoptosis in a rabbit model of disc degeneration." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 386012, (2015) (PubMed).

Chen, Yan, Wang, Yu, Wang, Zhang, Zhang, Zheng, Zhao, Zheng: "In vivo and in vitro evaluation of effects of Mg-6Zn alloy on apoptosis of common bile duct epithelial cell." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, (2014) (PubMed).

Chen, Luo, Liu, Yuan, Gu, Yang, Li, Tan: "Label-free electrochemical immunoassay of Bcl-2 protein expression on tumor cells." in: Talanta, Vol. 132, pp. 479-85, (2014) (PubMed).

Wang, Ma, Su: "NF-?B pathway contributes to cadmium-induced apoptosis of porcine granulosa cells." in: Biological trace element research, Vol. 153, Issue 1-3, pp. 403-10, (2013) (PubMed).

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background:

Synonyms: Bcl-2, PPP1R5, Apoptosis regulator Bcl-2, BCL2

Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling