Insulin antibody (AA 1-110)

Catalog No. ABIN7115273

The Mouse Monoclonal anti-Insulin antibody has been validated for IHC and ELISA. It is suitable to detect Insulin in samples from Human and Mouse.

Quick Overview for Insulin antibody (AA 1-110) (ABIN7115273)

Target: Insulin (INS)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: 0H2

Product Details anti-Insulin Antibody

Binding Specificity: AA 1-110

Purpose: INS antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-110aa

Immunogen: insulin

Isotype: IgG2a

Application Details

Application Notes: IHC: 1:500-1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: INS

Background:

Synonyms: Insulin|Insulin B chain|Insulin A chain|INS

Background: Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A-and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus. Insulin may also exist 36 kDa form corresponding to the hexameric insulin form.

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation