IL-33 antibody (AA 1-270)

Catalog No. ABIN7115461

This Mouse Monoclonal antibody specifically detects IL-33 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for IL-33 antibody (AA 1-270) (ABIN7115461)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 4E8

Product Details anti-IL-33 Antibody

Binding Specificity: AA 1-270

Purpose: IL33 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-270aa

Immunogen: interleukin 33

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL33

Background:

Synonyms: Interleukin-33 (IL-33)|Interleukin-1 family member 11 (IL-1F11)|Nuclear factor from high endothelial venules (NF-HEV)|Interleukin-33 (95-270)|Interleukin-33 (99-270)|Interleukin-33 (109-270)|IL33|C9orf26|IL1F11|NFHEV

Background: Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells(PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury(PubMed:17853410, PubMed:18836528). In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets(PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation(PubMed:18787100).

Molecular Weight: 35 kDa

Gene ID: 90865

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response