KEAP1 antibody (AA 350-624)

Catalog No. ABIN7115610

This anti-KEAP1 antibody is a Mouse Monoclonal antibody detecting KEAP1 in WB, ELISA, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for KEAP1 antibody (AA 350-624) (ABIN7115610)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 9E2

Product Details anti-KEAP1 Antibody

Binding Specificity: AA 350-624

Purpose: KEAP1 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 350-624aa

Immunogen: kelch-like ECH-associated protein 1

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:1000, IHC: 1:50-1:500, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background:

Synonyms: Kelch-like ECH-associated protein 1|Cytosolic inhibitor of Nrf2 (INrf2)|Kelch-like protein 19|KEAP1|INRF2|KIAA0132|KLHL19

Background: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.

Molecular Weight: 55-70 kDa

Gene ID: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location