HLA-A antibody

Catalog No. ABIN7116012

The Rabbit Polyclonal anti-HLA-A antibody (ABIN7116012) specifically detects HLA-A in WB, IHC and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for HLA-A antibody (ABIN7116012)

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HLA-A Antibody

Purpose: HLA-A antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: A synthetic peptide

Immunogen: major histocompatibility complex, class I, A

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Alternative Name: HLA-A

Background:

Synonyms: HLA class I histocompatibility antigen, A alpha chain|Human leukocyte antigen A (HLA-A)|HLA-A|HLAA

Background: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

Molecular Weight: 44 kDa

Gene ID: 3105

UniProt: P04439, P30443

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response