MAGEA3 antibody

Catalog No. ABIN7116156

The Mouse Monoclonal anti-MAGEA3 antibody (Clone 4E9) (ABIN7116156) specifically detects MAGEA3 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for MAGEA3 antibody (ABIN7116156)

Target: MAGEA3 (Melanoma Antigen Family A, 3 (MAGEA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAGEA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 4E9

Product Details anti-MAGEA3 Antibody

Purpose: MAGEA3 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: melanoma antigen family A, 3

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MAGEA3

Target: MAGEA3 (Melanoma Antigen Family A, 3 (MAGEA3))

Alternative Name: MAGEA3

Background:

Synonyms: Melanoma-associated antigen 3|Antigen MZ2-D|Cancer/testis antigen 1.3 (CT1.3)|MAGE-3 antigen|MAGEA3|MAGE3

Background: Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme(E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Molecular Weight: 35-55 kDa

Gene ID: 4102

UniProt: P43357