MITF antibody

Catalog No. ABIN7116282

The Rabbit Polyclonal anti-MITF antibody (ABIN7116282) specifically detects MITF in WB, FACS, ELISA and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MITF antibody (ABIN7116282)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP)

Product Details anti-MITF Antibody

Purpose: MITF antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: microphthalmia-associated transcription factor

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background:

Synonyms: Microphthalmia-associated transcription factor|Class E basic helix-loop-helix protein 32 (bHLHe32)|MITF|BHLHE32

Background: Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences(E-boxes)(5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase(TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase(TYR) and tyrosinase-related protein 1(TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Molecular Weight: 60-65 kDa

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding