NGEF antibody

Catalog No. ABIN7116830

The Rabbit Polyclonal anti-NGEF antibody is suitable to detect NGEF in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IP.

Quick Overview for NGEF antibody (ABIN7116830)

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGEF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-NGEF Antibody

Purpose: NGEF antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: neuronal guanine nucleotide exchange factor

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IP: 1:500-1:1000, IHC: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for NGEF

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Alternative Name: NGEF

Background:

Synonyms: Ephexin-1|Eph-interacting exchange protein|Neuronal guanine nucleotide exchange factor|NGEF

Background: Acts as a guanine nucleotide exchange factor(GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension(By similarity).

Molecular Weight: 75 kDa

Gene ID: 25791

UniProt: Q8N5V2

Pathways: Neurotrophin Signaling Pathway