PHF2 antibody

Catalog No. ABIN7117376

The Rabbit Polyclonal anti-PHF2 antibody is suitable to detect PHF2 in samples from Human. It has been validated for WB, ELISA, IHC and IP.

Quick Overview for PHF2 antibody (ABIN7117376)

Target: PHF2 (PHD Finger Protein 2 (PHF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-PHF2 Antibody

Purpose: PHF2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: PHD finger protein 2

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:1000, IP: 1:500-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for PHF2

Target: PHF2 (PHD Finger Protein 2 (PHF2))

Alternative Name: PHF2

Background:

Synonyms: Lysine-specific demethylase PHF2|GRC5|PHD finger protein 2|PHF2|CENP-35|KIAA0662

Background: Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3(H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3(H3K4me3) at rDNA promoters and promotes expression of rDNA.

Molecular Weight: 100 kDa, 150 kDa

Gene ID: 5253

UniProt: O75151