PRMT1 antibody

Catalog No. ABIN7117926

This anti-PRMT1 antibody is a Rabbit Polyclonal antibody detecting PRMT1 in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for PRMT1 antibody (ABIN7117926)

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PRMT1 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: protein arginine methyltransferase 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for PRMT1

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Alternative Name: PRMT1

Background: Synonyms:HMT2, HRMT1L2, IR1B4 Background:Arginine methyltransferase that methylates(mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4'(H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma(IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor(ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine(5hmC)-binding activity(PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP-and/or TET1-dependent manner(PubMed:25284789).

Molecular Weight: 40-42 kDa

Gene ID: 3276

UniProt: Q99873