PIAS4 antibody

Catalog No. ABIN7117942

This Rabbit Polyclonal antibody specifically detects PIAS4 in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PIAS4 antibody (ABIN7117942)

Target: PIAS4 (Protein Inhibitor of Activated STAT, 4 (PIAS4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PIAS4 Antibody

Purpose: PIAS4 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: protein inhibitor of activated STAT, 4

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for PIAS4

Target: PIAS4 (Protein Inhibitor of Activated STAT, 4 (PIAS4))

Alternative Name: PIAS4

Background:

Synonyms: E3 SUMO-protein ligase PIAS4|PIASy|Protein inhibitor of activated STAT protein 4|Protein inhibitor of activated STAT protein gamma (PIAS-gamma)|PIAS4|PIASG

Background: Functions as an E3-type small ubiquitin-like modifier(SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

Molecular Weight: 60 kDa

Gene ID: 51588

UniProt: Q8N2W9

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Positive Regulation of Response to DNA Damage Stimulus