POFUT1 antibody

Catalog No. ABIN7117981

This Rabbit Polyclonal antibody specifically detects POFUT1 in WB, IHC, ELISA, IP and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for POFUT1 antibody (ABIN7117981)

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POFUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-POFUT1 Antibody

Purpose: POFUT1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: protein O-fucosyltransferase 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for POFUT1

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Alternative Name: POFUT1

Background:

Synonyms: GDP-fucose protein O-fucosyltransferase 1|Peptide-O-fucosyltransferase 1 (O-FucT-1)|POFUT1|FUT12|KIAA0180

Background: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors(AChRs).

Molecular Weight: 44 kDa

Gene ID: 23509

UniProt: Q9H488

Pathways: Notch Signaling, SARS-CoV-2 Protein Interactome