RBPJ antibody

Catalog No. ABIN7118354

The Mouse Monoclonal anti-RBPJ antibody has been validated for WB and ELISA. It is suitable to detect RBPJ in samples from Human.

Quick Overview for RBPJ antibody (ABIN7118354)

Target: RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBPJ antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2A10

Product Details anti-RBPJ Antibody

Purpose: RBPJ antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: recombination signal binding protein for immunoglobulin kappa J region

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RBPJ

Target: RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))

Alternative Name: RBPJ

Background:

Synonyms: Recombining binding protein suppressor of hairless|CBF-1|J kappa-recombination signal-binding protein|RBP-J kappa (RBP-J, RBP-JK)|Renal carcinoma antigen NY-REN-30|RBPJ|IGKJRB|IGKJRB1|RBPJK|RBPSUH

Background: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins(Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions(4 % oxygen)(PubMed:23303788).

Molecular Weight: 60 kDa

Gene ID: 3516

UniProt: Q06330

Pathways: Notch Signaling, Stem Cell Maintenance, Smooth Muscle Cell Migration