RFC4 antibody

Catalog No. ABIN7118400

The Rabbit Polyclonal anti-RFC4 antibody (ABIN7118400) specifically detects RFC4 in WB, ELISA, IHC, IF and IP. The antibody is reactive with Human and Rat samples.

Quick Overview for RFC4 antibody (ABIN7118400)

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-RFC4 Antibody

Purpose: RFC4 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: replication factor C (activator 1) 4, 37kDa

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:100, IP: 1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RFC4

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Alternative Name: RFC4

Background:

Synonyms: Replication factor C subunit 4|Activator 1 37 kDa subunit (A1 37 kDa subunit)|Activator 1 subunit 4|Replication factor C 37 kDa subunit (RF-C 37 kDa subunit, RFC37)|RFC4

Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Molecular Weight: 38 kDa

Gene ID: 5984

UniProt: P35249

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA