RRP8 antibody

Catalog No. ABIN7118623

This Rabbit Polyclonal antibody specifically detects RRP8 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for RRP8 antibody (ABIN7118623)

Target: RRP8 (Ribosomal RNA Processing 8 (RRP8))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRP8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RRP8 Antibody

Purpose: RRP8 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ribosomal RNA processing 8, methyltransferase, homolog(yeast)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RRP8

Target: RRP8 (Ribosomal RNA Processing 8 (RRP8))

Alternative Name: RRP8

Background:

Synonyms: Ribosomal RNA-processing protein 8|Cerebral protein 1|Nucleomethylin|RRP8|KIAA0409|NML

Background: Essential component of the eNoSC(energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9'(H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.

Molecular Weight: 51 kDa

Gene ID: 23378

UniProt: O43159