RBX1 antibody

Catalog No. ABIN7118665

This Rabbit Polyclonal antibody specifically detects RBX1 in WB, IHC, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RBX1 antibody (ABIN7118665)

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-RBX1 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ring-box 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: RBX1

Background: Synonyms:RNF75, ROC1 Background:E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

Molecular Weight: 12 kDa

Gene ID: 9978

UniProt: P62877

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome