SIRT5 antibody

Catalog No. ABIN7119113

The Rabbit Polyclonal anti-SIRT5 antibody is suitable to detect SIRT5 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IP.

Quick Overview for SIRT5 antibody (ABIN7119113)

Target: SIRT5 (Sirtuin 5 (SIRT5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-SIRT5 Antibody

Purpose: SIRT5 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: sirtuin(silent mating type information regulation 2 homolog) 5

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IP: 1:500-1:1000, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for SIRT5

Target: SIRT5 (Sirtuin 5 (SIRT5))

Alternative Name: SIRT5

Background:

Synonyms: NAD-dependent protein deacylase sirtuin-5, mitochondrial|Regulatory protein SIR2 homolog 5|SIR2-like protein 5|SIRT5|SIR2L5

Background: NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins(PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting(PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species(PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2(By similarity). Has weak NAD-dependent protein deacetylase activity, however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c(CYCS) and a number of other proteins in vitro such as UOX.

Molecular Weight: 33 kDa

UniProt: Q9NXA8

Pathways: SARS-CoV-2 Protein Interactome