SREBF chaperone antibody

Catalog No. ABIN7119463

This Rabbit Polyclonal antibody specifically detects SREBF chaperone in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for SREBF chaperone antibody (ABIN7119463)

Target: SREBF chaperone (SCAP)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF chaperone antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SREBF chaperone Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: SREBF chaperone

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for SREBF chaperone

Target: SREBF chaperone (SCAP)

Alternative Name: SCAP

Background: Synonyms:KIAA0199 Background:Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway(By similarity).SCAP has 4 isoforms with MW 140, 98, 96 and 85 kDa (refer to UniProt: Q12770).

Molecular Weight: 140 kDa,96 kDa

Gene ID: 22937

UniProt: Q12770

Pathways: SARS-CoV-2 Protein Interactome