TOP3A antibody

Catalog No. ABIN7120017

The Rabbit Polyclonal anti-TOP3A antibody (ABIN7120017) specifically detects TOP3A in WB, ELISA and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TOP3A antibody (ABIN7120017)

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-TOP3A Antibody

Purpose: TOP3A antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: topoisomerase(DNA) III alpha

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TOP3A

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Alternative Name: TOP3A

Background:

Synonyms: DNA topoisomerase 3-alpha|DNA topoisomerase III alpha|TOP3A|TOP3

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.

Molecular Weight: 100-110 kDa

Gene ID: 7156

UniProt: Q13472

Pathways: DNA Damage Repair