TOP3A antibody (AA 350-430)

Catalog No. ABIN7226600

The Rabbit Polyclonal anti-TOP3A antibody is suitable to detect TOP3A in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for TOP3A antibody (AA 350-430) (ABIN7226600)

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TOP3A Antibody

Binding Specificity: AA 350-430

Purpose: Rabbit Anti-Topo IIIα Polyclonal Antibody

Specificity: Topo IIIα Polyclonal Antibody detects endogenous levels of Topo IIIα protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Topo IIIalpha at AA range: 350-430

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TOP3A

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Alternative Name: Topo IIIalpha

Background: TOP3A, TOP3, DNA topoisomerase 3-alpha, DNA topoisomerase III alphaTOP3A(topoisomerase(DNA) III alpha) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 115kD

Gene ID: 7156

UniProt: Q13472

Pathways: DNA Damage Repair