VPS4A antibody

Catalog No. ABIN7120723

This Rabbit Polyclonal antibody specifically detects VPS4A in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for VPS4A antibody (ABIN7120723)

Target: VPS4A (Vacuolar Protein Sorting-Associated Protein 4A (VPS4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-VPS4A Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: vacuolar protein sorting 4 homolog A(S. cerevisiae)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for VPS4A

Target: VPS4A (Vacuolar Protein Sorting-Associated Protein 4A (VPS4A))

Alternative Name: VPS4A

Background: Synonyms:FLJ22197, hVPS4, Protein SKD2, SKD1, SKD2, VPS4, VPS4 1, VPS4A Background:Involved in late steps of the endosomal multivesicular bodies(MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles(ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding(HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission(PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan(PubMed:22660413).

Molecular Weight: 49 kDa

Gene ID: 27183

UniProt: Q9UN37

Pathways: Microtubule Dynamics, CXCR4-mediated Signaling Events