ZEB1 antibody (AA 391-739)

Catalog No. ABIN7120942

This Rabbit Polyclonal antibody specifically detects ZEB1 in WB, ELISA, IHC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ZEB1 antibody (AA 391-739) (ABIN7120942)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-ZEB1 Antibody

Binding Specificity: AA 391-739

Purpose: ZEB1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 391-739aa

Immunogen: zinc finger E-box binding homeobox 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:200-1:2000,IHC: 1:500-1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1

Background:

Synonyms: Zinc finger E-box-binding homeobox 1|NIL-2-A zinc finger protein|Negative regulator of IL2|Transcription factor 8 (TCF-8)|ZEB1|AREB6|TCF8

Background: Acts as a transcriptional repressor. Inhibits interleukin-2(IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition(EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box(5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.

Molecular Weight: 210 kDa

Gene ID: 6935

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation