IRF9 antibody

Catalog No. ABIN7504160

This anti-IRF9 antibody is a Mouse Monoclonal antibody detecting IRF9 in IF and FACS. Suitable for Human.

Quick Overview for IRF9 antibody (ABIN7504160)

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRF9 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-IRF9 Antibody

Specificity: Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Cross-Reactivity (Details): Human

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human IRF9 protein

Isotype: IgG2a

Application Details

Application Notes:

Positive Control: MCF-7, Jurkat or K562 cells.

Known Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for IRF9

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Alternative Name: IRF9

Background: IFN alpha responsive transcription factor subunit, Interferon regulatory factor 9, Interferon-stimulated gene factor 3 gamma, interferon-stimulated transcription factor 3, gamma 48 kDa, IRF-9, ISGF-3 gamma, ISGF3 p48 subunit, ISGF3G, p48, Transcriptional regulator ISGF3 subunit gamma,Interferon regulatory factor 9 / IRF-9
Cellular localisation: Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

Molecular Weight: 43.34kDa

Gene ID: 10379, 1706

UniProt: Q00978

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Hepatitis C