CDX1 antibody

Catalog No. ABIN7504134

Product Details anti-CDX1 Antibody

Target: CDX1 (Caudal Type Homeobox 1 (CDX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDX1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The members of the murine Cdx family (Cdx1, Cdx2, and Cdx4) are members of the caudal-type homeobox family of genes, which are homologues of the Drosophila caudal gene required for anterior-posterior regional identity. The intestine-specific transcription factors Cdx1 and Cdx2 are candidate genes for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. The relative expression of Cdx1 to Cdx2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation. Cdx1 positively regulates its own expression. Cdx1 and Cdx2 are expressed in the small intestine and colon of fetus and adult. A decrease in human Cdx1 and/or Cdx2 expression is associated with colorectal tumorigenesis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways. The human Cdx1 gene maps to chromosome 5q32 and encodes a 265-amino acid protein.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length CDX1 protein

Isotype: IgG1

Application Details

Application Notes:

Positive Control: HT29 cells. Colon Carcinoma

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for CDX1

Target: CDX1 (Caudal Type Homeobox 1 (CDX1))

Alternative Name: CDX1 (CDX1 Products)

Synonyms: Cdx antibody, Cdx-1 antibody, cdx-1 antibody, xcad2 antibody, xtcad2 antibody, CDX-A antibody, CDXA antibody, CHOX-CAD1 antibody, CHox-cad antibody, CDX1 antibody, xCAD2 antibody, zgc:165581 antibody, cdx2 antibody, caudal type homeobox 1 antibody, caudal type homeo box 1 antibody, caudal type homeobox 1 S homeolog antibody, caudal type homeobox 1 b antibody, caudal type homeobox 1a antibody, CDX1 antibody, Cdx1 antibody, cdx1 antibody, cdx1.S antibody, cdx1b antibody, cdx1a antibody

Background: Caudal type homeobox 1, Caudal type homeobox transcription factor 1, Caudal-type homeobox protein 1,CDX1,CDX1 / Caudal Type Homeobox 1 (Intestine-Specific Transcription Factor)
Cellular localisation: Nuclear

Molecular Weight: 28kDa

Gene ID: 1044, 1545

UniProt: P47902