Mesothelin antibody (AA 273-407)

Catalog No. ABIN7126165

The Mouse Monoclonal anti-Mesothelin antibody (Clone MSLN-3384) (ABIN7126165) specifically detects Mesothelin in IHC. The antibody is reactive with Human samples.

Quick Overview for Mesothelin antibody (AA 273-407) (ABIN7126165)

Target: Mesothelin (MSLN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSLN-3384

Product Details anti-Mesothelin Antibody

Binding Specificity: AA 273-407

Purpose: Mesothelin (Mesothelial Marker) Antibody

Specificity: Mesothelin is a 40 kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69 kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: CAPAN-2 or HeLa cells. Lung Mesothelioma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: MSLN

Background:

CAK1, Megakaryocyte potentiating factor, Mesothelin, MSLN, SMR, SMRP

CAPAN-2 or HeLa cells. Lung Mesothelioma.

Molecular Weight: 40kDa (Mature form), 69kDa (Precursor)

Gene ID: 10232, 408488

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process