Recombinant Podoplanin antibody (AA 1-100)

Catalog No. ABIN7126197

The Rabbit Monoclonal anti-Podoplanin antibody is suitable to detect Podoplanin in samples from Human. It has been validated for ELISA, IF, IHC (f) and Coat.

Quick Overview for Recombinant Podoplanin antibody (AA 1-100) (ABIN7126197)

Target: Podoplanin (PDPN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Product Details anti-Podoplanin Antibody

Binding Specificity: AA 1-100

Specificity: It recognizes a muco-protein of 38-43 kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues within aa1-100 of Podoplanin

Isotype: IgG

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, cervical or lung carcinoma tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: PDPN

Background: Aggrus, Glycoprotein 36 KD, Glycoprotein 36, gp36, GP38, GP40, HT1A1, hT1alpha1, hT1alpha2, Lung type I cell membrane associated glycoprotein, Lung type I cell membrane associated glycoprotein T1A 2, OTS8,Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker)
Cellular localisation: Cell Surface

Molecular Weight: 17kDa

Gene ID: 10630, 468675

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport