CHEK2 antibody

Catalog No. ABIN7126216

This anti-CHEK2 antibody is a Mouse Monoclonal antibody detecting CHEK2 in WB, IF, IP and FACS. Suitable for Human.

Quick Overview for CHEK2 antibody (ABIN7126216)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-CHEK2 Antibody

Specificity: Recognizes a serine/threonine protein kinase that is a required check-point to mediate cell cycle arrest, activation of DNA repair and apoptosis. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. Also, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

Cross-Reactivity (Details): Human. Predicted to react with Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human CHEK2 corresponding to protein domain

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), ,Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HCT 116 cells. Human testis, spleen or colon.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHEK2

Background: Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHK2 checkpoint homolog (S. pombe), hCds1, HuCds 1, LFS2, PP1425, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2,CHEK2
Cellular localisation: Nucleus.

Molecular Weight: 60.91kDa

Gene ID: 11200, 291363, 505297

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle