Clusterin antibody (AA 150-300)
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- Target See all Clusterin (CLU) Antibodies
- Clusterin (CLU)
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Binding Specificity
- AA 150-300
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Clusterin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, i± and i², are associated through disulfide bonds. The i² subunit (also called ApoJi±) corresponds to residues 23-227. The i± subunit (also called ApoJi²) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences i²-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa150-300) of human CLU protein (exact sequence is proprietary)
- Isotype
- IgG2a
- Top Product
- Discover our top product CLU Primary Antibody
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- Application Notes
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Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human pancreas, tonsil or cervix.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Clusterin (CLU)
- Alternative Name
- CLU (CLU Products)
- Synonyms
- LOC100136120 antibody, clu antibody, MGC53055 antibody, zgc:73352 antibody, xclu antibody, MGC89163 antibody, CLU antibody, APO-J antibody, APOJ antibody, CLI antibody, CLU1 antibody, CLU2 antibody, KUB1 antibody, NA1/NA2 antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, TRPM-2 antibody, TRPM2 antibody, DAG antibody, RATTRPM2B antibody, SP40 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, AI893575 antibody, ApoJ antibody, Cli antibody, D14Ucla3 antibody, Sgp-2 antibody, Sgp2 antibody, Sugp-2 antibody, GP80 antibody, gpIII antibody, 40 antibody, clusterin-1 antibody, clusterin L homeolog antibody, clusterin antibody, Clusterin antibody, LOC100136120 antibody, clu.L antibody, clu antibody, CLU antibody, clus antibody, Clu antibody, LOC101113728 antibody
- Background
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Aging associated protein 4, Apolipoprotein J, CLU1, CLU2, Clusterin alpha / beta chain, Complement associated protein SP40, Complement cytolysis inhibitor a chain, NA1/NA2, D14Ucla3, Dimeric acid glycoprotein, GP80, Ku70-binding protein 1 (KUB1), Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B),Clusterin / Apolipoprotein J (APO-J)
Cellular localisation: Secreted. Can retrotranslocate from secretory compartments to cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytopla - Molecular Weight
- 70kDa (precursor), 36-39kDa (i±), 34-36kDa (i²)
- Gene ID
- 1191, 436657
- UniProt
- P10909
- Pathways
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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