Recombinant Desmin antibody

Catalog No. ABIN7126293

The Mouse Monoclonal anti-Desmin antibody is suitable to detect Desmin in samples from Human, Rat, Mouse, Chicken and Hamster. It has been validated for WB and IHC.

Quick Overview for Recombinant Desmin antibody (ABIN7126293)

Target: Desmin (DES)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse, Chicken, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RDES-1711

Product Details anti-Desmin Antibody

Purpose: Recombinant Desmin (Muscle Cell Marker) Antibody

Specificity: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain °™-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Cross-Reactivity (Details): Human, Mouse, Rat, Hamster and Chicken.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant full-length human desmin protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: SJRH30 cells. Uterus or Leiomyosarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: DES

Background:

CMD1I, CSM1, CSM2, DES, Intermediate filament protein

SJRH30 cells. Human uterus or leiomyosarcoma. Heart, Intestine or RH30.

Molecular Weight: 52kDa

Gene ID: 1674, 594952

UniProt: P17661