Recombinant TdT antibody (AA 1-100)

Catalog No. ABIN7126304

This Mouse Monoclonal antibody specifically detects TdT in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant TdT antibody (AA 1-100) (ABIN7126304)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TdT antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Clone: RDNTT-6909

Product Details anti-TdT Antibody

Binding Specificity: AA 1-100

Purpose: Recombinant TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker) Antibody

Specificity: Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL).

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa1-100) of human DNTT protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human thymus. Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: DNTT

Background:

Deoxynucleotidyltransferase terminal, DNTT, Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase, TDT, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal deoxyribonucleotidyltransferase

Human thymus. Jurkat cells.

Molecular Weight: 58kDa

Gene ID: 1791, 534206

UniProt: P04053

Pathways: DNA Damage Repair