SIRT2 antibody

Catalog No. ABIN7126393

The Mouse Monoclonal anti-SIRT2 antibody (Clone PCRP-SIRT2-1A8) (ABIN7126393) specifically detects SIRT2 in WB, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for SIRT2 antibody (ABIN7126393)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-SIRT2-1A8

Product Details anti-SIRT2 Antibody

Purpose: SIRT2 Antibody

Specificity: The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in a NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT proteins may function via mono-ADP-ribosylation of proteins. SIRT2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human SIRT2 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7 or U-87 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2

Background:

NAD-dependent deacetylase sirtuin-2, NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, Silencing information regulator 2 like, Silent information regulator 2, SIR2, SIR2 like protein 2, Sirtuin type 2

HeLa, MCF-7 or U-87 cells.

Molecular Weight: 43kDa

Gene ID: 22933, 466693

UniProt: Q8IXJ6