GLUL antibody (AA 50-250)

Catalog No. ABIN7126480

The Mouse Monoclonal anti-GLUL antibody (Clone GLUL-6600) (ABIN7126480) specifically detects GLUL in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow and Horse samples.

Quick Overview for GLUL antibody (AA 50-250) (ABIN7126480)

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Reactivity: Human, Mouse, Rat, Cow, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLUL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: GLUL-6600

Product Details anti-GLUL Antibody

Binding Specificity: AA 50-250

Purpose: Glutamine Synthetase / GLUL Antibody

Specificity: Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Cross-Reactivity (Details): Human. Horse. Cow.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human brain, stomach or thyroid. A-431 or Y79 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GLUL

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Alternative Name: GLUL

Background:

cell proliferation-inducing protein 59, GLUL, Glutamate ammonia ligase, GS, PIG43, PIG59, Glutamate decarboxylase, glutamine synthase, Glutamine synthetase, glutamine synthetase I, Proliferation inducing protein 43, GLNA, GLNS

Human Liver, Mouse Liver, Rat Liver, Human Brain, Mouse Brain, Rat Brain and Y79

Molecular Weight: 42kDa

Gene ID: 2752, 518525

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion