Recombinant MSH6 antibody (AA 1-100)

Catalog No. ABIN7126493

This Rabbit Monoclonal antibody specifically detects MSH6 in WB, IHC and ELISA. It exhibits reactivity toward Human, Dog and Cat.

Quick Overview for Recombinant MSH6 antibody (AA 1-100) (ABIN7126493)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog, Cat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MSH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: MSH6-6654R

Product Details anti-MSH6 Antibody

Binding Specificity: AA 1-100

Purpose: Recombinant MSH6 (DNA Mismatch Repair Protein) Antibody

Specificity: MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H). It has been suggested that the deficiencies in DNA mismatch repair protein(s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI). MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsiblefor the MSI.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determin

Positive_Control: HCT116, MCF-7 or HeLa cells. Human colon carcinoma or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6

Background:

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein

HCT116, MCF-7 or HeLa cells. Human colon carcinoma or tonsil.

Molecular Weight: 153kDa

Gene ID: 2956, 445052

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response