IGF1R antibody (AA 550-750)

Catalog No. ABIN7126548

The Mouse Monoclonal anti-IGF1R antibody has been validated for IF, FACS, Neut, AcA and in vitro. It is suitable to detect IGF1R in samples from Human.

Quick Overview for IGF1R antibody (AA 550-750) (ABIN7126548)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1R antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Neutralization (Neut), Activity Assay (AcA), In vitro Assay (in vitro)

Product Details anti-IGF1R Antibody

Binding Specificity: AA 550-750

Specificity: Receptor tyrosine kinases (RTKs) are transmembrane molecular scaffolds that influence cellular processes including the cell cycle, cell migration, cell metabolism, cell survival, proliferation and differentiation. Insulin-like growth factor-I receptor (IGF-IR) is an RTK that stimulates growth in many different cell types, blocks apoptosis, acts as an intermediate of many growth hormone responses and may stimulate the growth of some types of cancer. The IGF-IR cognate ligand Insulin-like growth factor-I (IGF-I) promotes association of IGF-IR with Shc, GRB2 and Sos 1, which initiates Ras and ERK kinase cascades, thereby modifying transcription factor activity, such as activation of the Elk transcription factors. The modular phosphotyrosine binding (PTB) domains of Insulin receptor substrate (IRS)-1 and -2 can associate with active IGF-IR and initiate phosphatidylinositol 3-kinase-dependent downstream signals. The human IGF-IR gene maps to chromosome 15q26.3 and encodes a 1,376 amino acid precursor protein that cleaves into i± and i² subunits. The human IGF-IIR gene maps to chromosome 6q26 and encodes a 2,491 amino acid transmembrane protein.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa550-750) of human IGF1R protein (exact sequence is proprietary)

Isotype: IgG2

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-4 μg/mL), In-vitro Neutralization of Biological Activity of IGF-1 (Order Ab without Azide)Optimal dilution for a specific application should be determined.

Positive_Control: Human breast carcinoma or colon carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF1R

Background: CD221 antigen, IGF 1 receptor, IGF 1R, IGFIR, IGFIRC, IGFR, Insulin like growth factor 1 receptor precursor, Insulin-like growth factor 1 receptor beta chain, JTK13, Soluble IGF1R variant 1, Soluble IGF1R variant 2,IGF1R / CD221
Cellular localisation: Cell membrane.

Molecular Weight: 130kDa (IGF-1R), 97kDa (IGF-1R), 200kDa (pro-IGF-1R)

Gene ID: 3480, 643120, 714012

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy