IL-2 antibody (AA 21-153)

Catalog No. ABIN7126565

This Mouse Monoclonal antibody specifically detects IL-2 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for IL-2 antibody (AA 21-153) (ABIN7126565)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-IL-2 Antibody

Binding Specificity: AA 21-153

Specificity: Interleukin-2 (IL-2) is a T cell stimulatory cytokine that induces T cell proliferation and NK cell proliferation and activation. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL-2 induces CTLA-4 and also functions as a survival factor for lymphocytes.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 21-153) of human IL2 protein (exact sequence is proprietary)

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human thymus or tonsil tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Background: Aldesleukin, Involved in regulation of T cell clonal expansion, Lymphokine, POIL2, T-cell growth factor (TCGF),Interleukin-2 (IL-2)
Cellular localisation: Secreted.

Molecular Weight: 15kDa

Gene ID: 3558, 89679

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation