ITGB1 antibody

Catalog No. ABIN7126605

The Mouse Monoclonal anti-ITGB1 antibody has been validated for ELISA, IHC (f) and Coat. It is suitable to detect ITGB1 in samples from Human.

Quick Overview for ITGB1 antibody (ABIN7126605)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Product Details anti-ITGB1 Antibody

Specificity: Recognizes CD29. Binds to the cell membrane of T- and B- lymphocytes, monocytes, platelets, fibroblasts, endothelial cells, mast cells and smooth muscle cells. CD29 is an adhesion receptor and co-stimulatory molecule. This antigen was initially characterized as GPIIa on platelets and as the common b1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin alpha subunits. It also mediates adhesion to invasion and thrombospondin.

Cross-Reactivity (Details): Human. Does not react with Mouse or Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified human beta 1 integrin from HT1080 fibrosarcoma cell extract.

Isotype: IgG1

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HT1080 whole cell lysate. HeLa cell line.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1

Background: Fibrinogen Receptor beta subunit, FNRB, Glycoprotein IIa (GPIIA), Integrin beta-1 (ITGB1), integrin VLA-4 beta subunit, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, VLA-4 subunit beta, VLA-BETA, VLAB,CD29 (Stem Cell Marker)
Cellular localisation: Cell Surface

Molecular Weight: 110kDa (non-reduced) & 130kDa (reduced)

Gene ID: 3688, 643813

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome