SMAD3 antibody

Catalog No. ABIN7126664

This Mouse Monoclonal antibody specifically detects SMAD3 in IF and FACS. It exhibits reactivity toward Human.

Quick Overview for SMAD3 antibody (ABIN7126664)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-SMAD3-1A2

Product Details anti-SMAD3 Antibody

Purpose: SMAD3 Antibody

Specificity: Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been implicated as downstream effectors of TGF æ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP-2 and BMP-4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF æ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF æ signaling by interfering with TGF æ-mediated phosphorylation of other Smad family members.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human SMAD3 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background:

hMAD-3, hSMAD3, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MADH3, SMA and MAD related protein 3, SMAD, mothers against DPP homolog 3

HeLa or HepG2 cells.

Molecular Weight: 54kDa

Gene ID: 4088, 727986

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy