Recombinant CD99 antibody (C-Term)

Catalog No. ABIN7126690

The Rabbit Monoclonal anti-CD99 antibody (Clone MIC2-3478R) (ABIN7126690) specifically detects CD99 in IHC and WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant CD99 antibody (C-Term) (ABIN7126690)

Target: CD99

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: MIC2-3478R

Product Details anti-CD99 Antibody

Binding Specificity: C-Term

Purpose: Recombinant CD99 / MIC2 (Ewing s Sarcoma Marker) Antibody

Specificity: CD99 is a type I transmembrane glycoprotein encoded by the CD99 gene and the functions of CD99 in cells in which CD99 was highly expressed have been studied and they were as follows: cell death of thymocytes and T lymphocytes, migration through monocyte endothelial junctions by adhesion and diapedesis, cell-cell adhesion in lymphocytes, maintenance of cellular morphology in Hodgkin and Reed/Sternberg cells, and recruitment of T cells.CD99 expression has been reported in many cell types, such as hematopoietic cells, endothelial cells, central nervous system ependymal cells, thymocytes, granular cells of the ovary, Sertoli cells, and pancreatic islet cells. And in tumors it expressed by virtually almost all Ewing s sarcoma and primitive peripheral neuroectodermal tumors (ES/PNET) and demonstrates strong and diffuse membranous staining. Other tumors that may show CD99 expression include neuroendocrine carcinomas, mesenchymal chondrosarcomas, solitary fibrous tumors, synovial sarcomas, vascular tumors, small round blue cell tumors, lymphoblastic lymphoma, acute myeloid leukemia, and myeloid sarcoma. Studies have shown that CD99 may be a sensitive marker for Ewing s sarcoma and peripheral neuroectodermal tumors.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified by Protein A Column.

Immunogen: Synthetic peptide corresponding to CD99 residues within aa85 C terminal of CD99 was used as an immunogen

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4 cells. Pancreas or Ewing s sarcoma

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background:

12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2

MCF7, A549, MOLT-4 cells. Pancreas or Ewing s sarcoma

Molecular Weight: 27-32kDa

Gene ID: 4267, 653349

UniProt: P14209