MME antibody (AA 54-181)

Catalog No. ABIN7126706

This Mouse Monoclonal antibody specifically detects MME in IHC. It exhibits reactivity toward Human.

Quick Overview for MME antibody (AA 54-181) (ABIN7126706)

Target: MME (Membrane Metallo-Endopeptidase (MME))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MME antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MME-4232

Product Details anti-MME Antibody

Binding Specificity: AA 54-181

Purpose: CD10 (Membrane Metalloendopeptidase) Antibody

Specificity: Recognizes a 100 kDa glycoprotein, identified as CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CD10 protein fragment (aa 54-181) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil, small intestine or kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MME

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: MME

Background:

Atriopeptidase, Common acute lymphocytic leukemia antigen (CALLA), Enkephalinase (EPN), gp100, Membrane metalloendopeptidase (MME), Neprilysin, Neutral endopeptidase (NEP), Skin fibroblast elastase (SFE)

small intestine or kidney., Tonsil

Molecular Weight: 100kDa

Gene ID: 4311, 307734

UniProt: P08473

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration