Recombinant MYH11 antibody (AA 1-100)

Catalog No. ABIN7126733

This Rabbit Monoclonal antibody specifically detects MYH11 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant MYH11 antibody (AA 1-100) (ABIN7126733)

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MYH11 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MYH11-4337R

Product Details anti-MYH11 Antibody

Binding Specificity: AA 1-100

Purpose: Recombinant Smooth MuscleMyosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Antibody

Specificity: Smooth Muscle Myosin, heavy chain (SMM-HC) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early and is specific for smooth muscle development. SM-MHC stains the intact myoepithelial cell (MEC) layers present in benign and in situ malignant breast and bronchioloalveolar lesions and is therefore very helpful in distinguishing between benign and malignant tumors. The antibody reacts with smooth muscle cells and myoepithelial cells, but not with myofibroblasts. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human fragment corresponding to Myosin residues within aa1-100 of Myosin (Smooth Muscle)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human smooth muscle, uterus or breast.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Alternative Name: MYH11

Background:

AAT4, Myosin heavy chain 11 (MYH11), Myosin heavy chain 11 smooth muscle, Smooth muscle myosin heavy chain 11, Myosin-11, SM1, SM2, SMHC, SMMHC

Human smooth muscle, uterus or breast.

Molecular Weight: 227kDa

Gene ID: 4629, 460109

UniProt: P35749