Myogenin antibody

Catalog No. ABIN7126738

The Mouse Monoclonal anti-Myogenin antibody (Clone PCRP-MYOG-1C5) (ABIN7126738) specifically detects Myogenin in WB, ELISA, FACS and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Myogenin antibody (ABIN7126738)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myogenin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PCRP-MYOG-1C5

Product Details anti-Myogenin Antibody

Purpose: Myogenin / Myf-4 (Skeletal Muscle Marker) (Transcription Factor) Antibody

Specificity: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80 % amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Cross-Reactivity (Details): Human, Mouse andRat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human myogenin recombinant protein

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Rh-30 or HeLa cells. Human skeletal muscle or rhabdomyosarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: MYOG

Background:

bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Rh-30 or HeLa cells. Human skeletal muscle or rhabdomyosarcoma.

Molecular Weight: 34kDa

Gene ID: 4656, 2830

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development