NEUROD2 antibody

Catalog No. ABIN7126743

The Mouse Monoclonal anti-NEUROD2 antibody is suitable to detect NEUROD2 in samples from Human. It has been validated for ELISA, IF and FACS.

Quick Overview for NEUROD2 antibody (ABIN7126743)

Target: NEUROD2 (Neurogenic Differentiation 2 (NEUROD2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEUROD2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-NEUROD2-1G1

Product Details anti-NEUROD2 Antibody

Purpose: NEUROD2 (Neurogenic Differentiation 2) (Transcription Factor) Antibody

Specificity: Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes, both of which work together to activate DNA transcription. Class A proteins include the ubiquitously expressed E-box binding factors, namely E2A, ITF-2 and HEB, while class B proteins, such as MyoD, myogenin and Neuro D ( æ2), are transiently expressed and exhibit a much more limited tissue distribution. Working in opposition to these positively acting factors are a specialized group of basic helix-loop-helix(bHLH) transcription factorsthat function as dominant negative regulators and are involved in cell lineage determination and differentiation. Neuro D2 (neurogenic differentiation 2), also known as NDRF, NEUROD2 or bHLHa1, is a 382 amino acid nuclear protein that contains one bHLH domain and functions to induce neurogenic differentiation, playing an important role in the maintenance and determination of cell fate.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human NEUROD2 protein

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human brain.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NEUROD2

Target: NEUROD2 (Neurogenic Differentiation 2 (NEUROD2))

Alternative Name: NEUROD2

Background:

bHLHa1, class A basic helix loop helix protein 1, NDR2, NDRF, NeuroD-related factor, NeuroD2, neurogenic basic helix loop helix protein, neurogenic differentiation 2, Neurogenic differentiation factor 2, neuronal differentiation 2

HeLa cells. Human brain.

Molecular Weight: ~41kDa

Gene ID: 4761, 322431

UniProt: Q15784