Recombinant PAX5 antibody (AA 235-382)

Catalog No. ABIN7126757

This Rabbit Monoclonal antibody specifically detects PAX5 in WB and IHC. It exhibits reactivity toward Human and Dog.

Quick Overview for Recombinant PAX5 antibody (AA 235-382) (ABIN7126757)

Target: PAX5 (Paired Box 5 (PAX5))

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PAX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: PAX5-3977R

Product Details anti-PAX5 Antibody

Binding Specificity: AA 235-382

Purpose: Recombinant PAX5 / BSAP (Early B-Cell Marker) Antibody

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 21,000, full-length human proteins. The PAX5 gene is a member of thepaired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as thepaired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development andspermatogenesis.

Cross-Reactivity (Details): Human. Dog.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, spleen or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PAX5

Target: PAX5 (Paired Box 5 (PAX5))

Alternative Name: PAX5

Background:

B cell lineage specific activator, BSAP, EBB-1, KLP, Paired box 5

Human tonsil, Spleen or lymph node, K-562, Ramos

Molecular Weight: 42kDa

Gene ID: 5079, 654464

UniProt: Q02548