Recombinant PAX7 antibody (AA 301-505)

Catalog No. ABIN7126760

This Rabbit Monoclonal antibody specifically detects PAX7 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant PAX7 antibody (AA 301-505) (ABIN7126760)

Target: PAX7 (Paired Box 7 (PAX7))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: PAX7-7079R

Product Details anti-PAX7 Antibody

Binding Specificity: AA 301-505

Purpose: Recombinant PAX7 (Rhabdomyosarcoma Marker) Antibody

Specificity: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (aa301-505) of human PAX7 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human rhabdomyosarcoma (RMS) or testicular carcinoma.,

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background:

HUP1, Paired box gene 7, Paired box homeotic gene 7, Paired domain gene HuP1, PAX7 transcriptional factor, PAX7/FKHR fusion gene, PAX7B, RMS2

Human rhabdomyosarcoma (RMS) or testicular carcinoma.

Molecular Weight: 57kDa

Gene ID: 5081, 113253

UniProt: P23759